Fig. 7

AG205 does not affect decidualization. The influence of AG205 on T-HESCs viability was performed after the cells were incubated with indicated concentrations of AG025 for 10 days and analyzed with colorimetric assay (A). The absorbance values for cultures with AG205 were compared to the DMSO control (0 µM). The PRL mRNA expression levels were analyzed after cells were cultured with (black line) or without (red line) 15 µM AG205 (B). Results are shown as the mean ± SEM from three independent biological replicates. Statistical analysis was performed by two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The cellular morphology changes of T-HESCs were imaged with microscopy in the bright field when cells were cultured without (upper panel) or with (down panel) MPA/cAMP upon 15 µM AG205 treatment (C). Scale bar: 200 µm. The PGRMC1 protein expression changes in T-HESCs were analyzed by western blot when cells were treated with DMSO (left panel) or 15 µM AG205 (right panel) upon decidualization induction (D). β-actin was used as a loading control. The interactions between PGRMC1 and PHB1 (E) or PHB2 (F) in T-HESCs were analyzed by proximity ligation assay when cells were cultured with 15 µM AG205 upon decidualization induction. Each red spot represents a single interaction. Nuclear stain: DAPI. Magnification 40 ×