Fig. 4

Prei-nuclear PGRMC1-signal increased during decidualization. (A): Immunofluorescence staining of PGRMC1 in T-HESCs treated with DMSO (left) as control or MPA/cAMP (right) for 10 days decidualization induction. PGRMC1 shows in red and the nucleus was stained with DAPI in blue. Scale bar: 200 µm. (B) Analysis of PGRMC1 localization by subcellular fractionation in T-HESCs treated with DMSO (left) or MPA/cAMP (right), measured by western blot. PGRMC1 was immunoblotted in equal amounts of cytoplasmic (CE), membrane (ME), and nuclear (NE) biomaterial. Compartment-specific markers: Calreticulin (55 kDa), β-actin (47 kDa), and Histon H3 (17 kDa) were used as loading controls for the membrane, cytoplasmic, and nuclear fractions, respectively