Fig. 4
Knockdown of RSAD2 rescues DGE observed in insulin + PIK3CA H1047R. A, Volcano plot of DGE following insulin treatment in siRSAD2 cells relative to vehicle, FDR < 0.001. Significant genes are labeled in red. B, Euler diagram, overlapping DEG in Insulin vs. Control, siRSAD2 + Insulin vs. siRSAD2 and siRSAD2 + PIK3CAH1047R + Insulin vs. siRSAD2 + PIK3CAH1047R. Statistic is hypergeometric enrichment. C, Volcano plot of DGE following insulin treatment in siRSAD2 and PIK3CAH1047R cells relative to vehicle, FDR < 0.001. Significant genes red. D, Euler diagram of overlapping DEG in PIK3CAH1047R + Insulin vs. PIK3CAH1047R and siRSAD2 + PIK3CAH1047R + Insulin vs. siRSAD2 + PIK3CAH1047R. Statistic is hypergeometric enrichment. E, GOBP enrichment among genes differentially expressed in PIK3CAH1047R + Insulin vs. PIK3CAH1047R but not siRSAD2 + PIK3CAH1047R + Insulin vs. siRSAD2 + PIK3CAH1047R. F, Euler diagram of overlapping DEG in PIK3CAH1047R + Insulin vs. PIK3CAH1047R and siRSAD2 vs. Control. Statistic is hypergeometric enrichment. G, Pie chart displaying direction of DGE for overlapping genes from J, and directionality in siRSAD2 vs. Control (listed first) and PIK3CAH1047R + Insulin vs. PIK3CAH1047R (listed second). H, Fold-change values for overlapping genes from J in siRSAD2 vs. Control (x-axis) and PIK3CAH1047R + Insulin vs. PIK3CAH1047R (y-axis). Red dashed line is line of best fit. Statistic is Pearson’s correlation. I, GOBP enrichment among overlapping genes downregulated in both datasets (n = 483) as in K. J, GOBP enrichment among overlapping genes downregulated in siRSAD2 vs. Control and upregulated in PIK3CAH1047R + Insulin vs. PIK3CAH1047R (n = 102) as in K