Table 1 The characteristic of primer sequences used in real-time quantitative reverse transcription polymerase chain reaction assays
Genes | Primer sequence (5’-3’) | GenBank accession numbers | Product size (bp) |
|---|---|---|---|
FSH-R | F:ACAGGGTCTTCCTCTGCCAA R:GGTTGGAGAACACATCTGCC | 14,309 | 201 |
CYP19a1 | F:CGCAGAGTATCCAGAGGTCG R:CGCATGACCAAGTCCACAAC | 13,075 | 159 |
AMH | F:TTTGGTGCTAACCGTGGACT R:CAGCGGGAATCAGAGCCAAA | 11,705 | 138 |
PCNA | F:GCACGTATATGCCGAGACCTT R:GACAGTGGAGTGGCTTTTGT | 18,538 | 233 |
BMP15 | F:CATCCAAGGGAGAACCGCAC R:AGTTGATGGCGGTAAACCACA | 12,155 | 176 |
GAPDH | F:ACTCTTCCACCTTCGATGCC R:TGGGATAGGGCCTCTCTTGC | 14,433 | 193 |
CYP11a1 | F:TATATTGGGCTGGGCAAGTGTTA R:AAAGTGCCCAGCTTCTCCCTATA | 13,070 | 236 |
CYP17a1 | F:GGCTAACATTGACTCCAGCATTG R:CTGGGTGTGGGTGTAATGAGATG | 13,074 | 171 |
STAR | F:AGCATGTTCCTCGCTACGTT R:ACCTCTCCCTGCTGGATGTA | 20,845 | 242 |
HSD3B1 | F:AGATGTTGGTGCAGGAGAAAGAA R:CAACACTGTCACCTTGGTCTTTG | 15,492 | 116 |