Fig. 2

Dnajb7 knockout mice were generated by CRISPR/Cas9 technology. A Schematic diagram of Dnajb7^−/− mouse creation. B Sanger sequencing of genomic DNA showing a 335-bp deletion in the Dnajb7 gene. *, premature stop codon. C Dnajb7^−/− mice were identified by genomic PCR. D In situ hybridization of Dnajb7 mRNA in adult Dnajb7^+/+ and Dnajb7^−/− testes. Dnajb7 mRNA was absent in adult Dnajb7^−/− testes but was detectable in adult Dnajb7^+/+ testes. Scale bar, 50 μm. E DNAJB7 protein was not detected in adult Dnajb7^−/− testes, n = 3 for each genotype