Fig. 2

Experimental setup. Cryopreserved ovarian cortex tissue from 20 patients was thawed and cut into two halves with one half receiving needle puncturing and the other half serving as the untreated control. The cortex pieces were xenografted to immunodeficient mice. Grafts was retrieved after 3, 6 and 10 days (N = 8) for immunohistochemistry and gene expression analysis, and after 4 weeks for histological analysis of follicle density and morphology. (a) CD31 staining after 10 days of xenografting from control group. Scale bar = 250 µm. (b) PAS-stained section from 4-week xenografted ovarian tissue showing representative follicles. Scale bar = 100 µm