Fig. 4
From: Insufficient HtrA2 causes meiotic defects in aging germinal vesicle oocytes
Knockdown of HtrA2 augments the incidence of aneuploidy in mouse oocytes. (A) Control and HtrA2-KD metaphase II oocytes were labeled with Hoechst 33342 to visualize the chromosomes (blue). Representative confocal images are shown. Scale bar: 10 μm. (B) Quantitative analysis of chromosome defects in control (n = 62) and HtrA2-KD (n = 119) oocytes. (C) Chromosome spreading of control and HtrA2-KD metaphase II oocytes. Chromosomes were stained with Hoechst 33342 (purple). (D) Quantification of aneuploidy in control (n = 43) and HtrA2-KD (n = 45) oocytes. Data are expressed as the mean ± SEM of three independent experiments. *, p < 0.05. HtrA2-KD, use siRNA-1