Fig. 2
From: Insufficient HtrA2 causes meiotic defects in aging germinal vesicle oocytes
HtrA2 knockdown activates the SAC in young oocytes. A Control and HtrA2-KD oocytes were stained with anti-BubR1 antibody (green) and counterstained with propidium iodide to visualize the chromosomes (red). Representative images of pre-metaphase I and metaphase I oocytes are shown. B Quantitative analysis of BubR1 fluorescence intensity in control (n = 25) and HtrA2-KD (n = 30) oocytes. Data are expressed as the mean ± SEM of three independent experiments. *, p < 0.05. Scale bar: 5 μm. HtrA2-KD, use siRNA-1