Fig. 1

High-fat diet results in body mass gain in CD-1 mice. A Heatmap representation of caloric contribution of protein, fat and carbohydrate for control diet and high-fat diet. B Experimental timeline. C Body mass of female mice on control diet (n = 9) and high-fat diet (n = 13) mice (grams) over time, beginning at week 0 of diet. Measurements represent mean ± standard deviation (S.D.). Statistic is two-tailed, unpaired t-test between two groups at same timepoint. D Relative body mass of mice on high-fat diet (n = 13) relative to mice on control diet (n = 9) within the same cohort, mean ± S.D. Statistic is two-tailed, unpaired t-test between two groups at same timepoint. E Average food consumption per day per mouse (grams), mean ± S.D. Measurements were made weekly. Statistic is two-tailed, paired t-test. F Gain in body mass after 18 weeks for each mouse relative to body mass at week 0 for control diet (n = 9) and high-fat diet (n = 13), mean ± S.D. Statistic is unpaired t-test. G, H Gross images of control diet and high-fat diet mice after 18 weeks on diet. Uterus is denoted with arrowhead in panel H. *p < 0.05; **p < 0.01; ***p < 0.001