Fig. 2

Authentication of the Padi2/Padi4 double knockout. a RT-PCR of salivary gland tissue shows that the DKO mice lack Padi2 transcripts. b PCR analysis of genomic DNA (15% polyacrylamide gel) confirms a 4bp deletion within the Padi4 gene of DKO mice. c Western blot analysis of spleen lysate confirms that DKO mice lack both PAD2 and PAD4 mature proteins. d RT-PCR of testicular tissue confirms transcription of both Padi1 and Padi3 is unaffected by the deletion of nearby genes