Fig. 7

Effect of prolactin on kisspeptin, NKB, and DynA expression in mHypoA-55 cells. (A) mHypoA-55 cells were stimulated with the indicated concentrations of prolactin for 24 h. Lysates (20  g protein) from mHypoA-55 cells were analyzed by SDS-PAGE followed by immunoblotting and incubation with antibodies against kisspeptin, NKB, and DynA. The bands were visualized using an HRP-conjugated secondary antibody