Fig. 6

Effect of NKB and DynA stimulation on Kiss-1 gene expression in mHypoA-55 cells. mHypoA-55 cells were stimulated with the indicated concentrations of NKB or DynA for 24 h. (A) After stimulation, mRNA was extracted and reverse transcribed, and Kiss-1 mRNA levels were measured by quantitative RT-PCR. Samples for each experimental group were run in duplicate and normalized to the mRNA levels of GAPDH as a housekeeping gene. The results are expressed as fold induction over unstimulated cells and presented as the mean ± SEM of three independent experiments