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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Hyperandrogenism induces proportional changes in the expression of Kiss-1, Tac2, and DynA in hypothalamic KNDy neurons

Fig. 5

Effect of DHT supplementation on Kiss-1, Tac3, and DynA gene expression in ovary-intact female rats. Six-week-old female rats were injected subcutaneously with DHT (5 mg/kg/day) daily for 7 days. After the rats were euthanized, hypothalamic tissues containing the ARC were removed from control (n = 4) and DHT-treated rats (n = 4), and mRNA was extracted from these tissues and reverse transcribed. Kiss-1, Tac3, and DynA mRNA levels were measured by quantitative RT-PCR. Samples for each experimental group were run in duplicate and normalized to the mRNA levels of GAPDH as a housekeeping gene. The results are expressed as fold induction over control and presented as the mean ± SEM. *P < 0.05 vs. control

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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