Fig. 4

Effect of DHT on the expression of kisspeptin, NKB, and DynA in mHypoA-55 cells. (A) mHypoA-55 cells were stimulated with the indicated concentrations of DHT for 24 h, and lysates (20  g protein) from the treated cells were analyzed by SDS-PAGE followed by immunoblotting and incubation with antibodies against kisspeptin, NKB, and DynA. The bands were visualized using an HRP-conjugated secondary antibody. Scanning densitometry of bands was performed using ImageJ to determine differences in kisspeptin (A), NKB (B), and DynA (C) expression, with normalization to β-actin levels. Results are expressed as fold stimulation over the unstimulated group/control. Values are the mean ± SEM of fold stimulation from independent experiments. **P < 0.01 vs. control