Fig. 3
From: Novel variants in helicase for meiosis 1 lead to male infertility due to non-obstructive azoospermia
Immunofluorescent staining of histological sections from the testis biopsy of the patient carrying the HFM1 variants using γH2AX (green), H3S10p (red), and Hoechst (blue). Scale bar represents 5 μm. A high density of metaphases was observed and almost all the metaphases were apoptotic. The organization of the apoptotic metaphases appeared to be more dispersed in samples of F1 II-1. In F2 II-1 patient rare metaphases were observed and all the metaphases displayed an aberrant pattern of γH2AX spots whereby γH2AX patches covered the entire nucleus