mRNA | PCR primer pair | Primers 5'-3' | Sequence | Annealing Temperature (°C) | Product size |
---|---|---|---|---|---|
LH/hCG | Outer pair | Forward | CAA TGT GAA AGC ACA GTA AGG A | 56 | Â |
 |  | Reverse | AGG CTA TGA GCA GCA GAT AGA G | 56 | 343 |
 | Inner pair | Forward | GAA CTG AGT GGC TGG GAC TA | 56 |  |
 |  | Reverse | GCA AAA GTC TGC AAA GGA GA | 56 | 249 |
HPRT | Outer pair | Forward | CTCCGCCTCCTCCTCTGCT | 50 | Â |
 |  | Reverse | GCCTGACCAAGGAAAGCAAAG | 50 | 528 |
 |  | Forward | GCCGGCTCCGTTATGGCG | 55 |  |
 | Inner pair | Reverse | AGCCCCCCTTGAGCACACAGA | 55 | 226 |