Skip to main content

Table 2 Oligonucleotide primers and TaqMan probes used for real-time RT-PCR analysis

From: Cloning and expression of two new prolactin-related proteins, prolactin-related protein-VIII and -IX, in bovine placenta

Gene

Primer or TaqMan probe

Sequence

Position

bPRP-VIII

Forward

5'-CAAGGGTCCTGCCTGCTG-3'

98–115

(AB196438)

Reverse

5'-GGCATGTTCTGCCTTGGC-3'

164–344

 

Probe

5'-TGCTGCTGGTCATGTCAAATCTGCTCC-3'

117–143

bPRP-IX

Forward

5'-ATATGCCCAGGGCAAACTGT-3'

287–306

(AB204881)

Reverse

5'-TCGGGAGCATGGAAGGAAT-3'

358-340

 

Probe

5'-TATCAATGCCACCAACAGCTGCCACA-3'

311–336

GAPDH

Forward

5'-AAGGCCATCACCATCTTCCA-3'

178–197

(U85042)

Reverse

5'-CCACTACATACTCAGCACCAGCAT-3'

253-230

 

Probe

5'-AGCGAGATCCTGCCAACATCAAGTGG-3'

200–225

  1. TaqMan probes of bPRP-VIII and -IX have 5'-FAM dye and 3'-TAMRA dye
  2. TaqMan probe of GAPDH has 5'-VIC dye and 3'-TAMRA dye