Figure 9

Production of recombinant bPRP-VIII, bPRP-IX, bPRP-I, and bPL proteins. Conditioned media from HEK 293 cells transiently transfected with each gene were collected, and the proteins (10 μg) were loaded in separate lanes. The proteins were separated by SDS PAGE and specific proteins were detected by Western blot analysis using an anti-FLAG tag. MW Marker: molecular weight marker. NC: Negative control (transfected vector only).