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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: A link between high serum levels of human chorionic gonadotrophin and chorionic expression of its mature functional receptor (LHCGR) in Down's syndrome pregnancies

Figure 5

At lease six LHCGR protein isoforms are expressed in human placenta. a) The HEK 293 (LHCGR ECD 1–362) cells grown in the absence (lane 1), in the presence of tetracycline (lane 2), 7 wk (lane 3) and 10 wk (lane 4) of gestation placental tissues were lysed with detergent (T-Per) and extracts (10 μg of protein in each lane) were immuno-reacted with LHR 29 antibody; b) Placental tissues (10–14 wks gestation) were extracted with Tri-zol reagents, proteins (10 μ/lane) were separated by extended electrophoresis in 8% SDS-PAGE and the blot was reacted with LHR29 antibody.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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